The stereochemical course at phosphorus of the reaction catalyzed by phosphoenolpyruvate carboxylase.
نویسندگان
چکیده
[(S)-16O,17O]Thiophosphoenolpyruvate has been used as a substrate in H218O for the reaction catalyzed by phosphoenolpyruvate carboxylase, and the absolute configuration of the product, inorganic [16O,17O,18O]thiophosphate, has been determined. The reaction proceeds with inversion of configuration at phosphorus, thus ruling out the cyclic mechanism that has been proposed for this enzyme. The stereochemical result is consistent with a stepwise mechanism involving the intermediate formation of carboxyphosphate.
منابع مشابه
Chirality at a pro-pro-prochiral phosphorus center. Stereochemical course of the 5'-nucleotidase-catalyzed reaction
Journal of the American Chemical Society is published by the American Chemical Society. 1155 Sixteenth Street N.W., Washington, DC 20036 Chirality at a pro-pro-prochiral phosphorus center. Stereochemical course of the 5'-nucleotidase-catalyzed reaction Ming-Daw Tsai, and Ting-Ting Chang J. Am. Chem. Soc., 1980, 102 (16), 5416-5418• DOI: 10.1021/ja00536a061 • Publication Date (Web): 01 May 2002 ...
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ورودعنوان ژورنال:
- The Journal of biological chemistry
دوره 257 24 شماره
صفحات -
تاریخ انتشار 1982